Fluorescent and 

Holographic Microscopy  

Services

• Sample preparation and processing expertise:
• • Tissue embedding and sectioning using a cryostat
  • Immunofluorescence and other stainings for cells and tissues
  • Cell culture and live cell-compatible imaging
  • Expansion microscopy protocol development
• Image acquisition:
• • Automated high-throughput acquisitions (slide or multi-well plate imaging)
  • High-resolution imaging (confocal, super-resolution)
  • High temporal resolution (spinning disk, resonant scanner)
• Data interpretation and analysis:
• • Qualitative interpretation of images for all types of biological samples, sub-cellular organization interpretation
  • Quantitative pipeline development
• Training in fluorescence microscopy techniques

Equipments

• Versatile wide-field fluorescent microscopes
• Confocal microscopes equipped with classic and resonant scanners (Stellaris 5 from Leica), spinning disk confocal microscope
• Super-resolution STED microscope (Stellaris 8 from Leica, equipped with FLIM module)
• FT-IR microscope (Hyperion II from Bruker)

 

Electron Microscopy 

Services

Sample preparation for SEM, TEM, and cryo-EM, and processing expertise:

• Sample preparation, sectioning (vibratome), and drying processes for SEM
• Cell and tissue embedding in resins for TEM
• Ultramicrotomy
• Tokuyasu technique (immunogold labeling)
• Negative staining and cryo-plunging (Vitrobot)

Image acquisition:

• SEM imaging at high vacuum or in low vacuum
• 100kV and 200kV RT-TEM imaging
• Single particle analysis (negative staining and in cryogenic conditions)
• RT and Cryo-Electron tomography
• STEM imaging and tomography

Data interpretation and analysis:

• Ultrastructure detail analysis for all types of biological samples (cells, tissues, etc.)
• Histopathological interpretation
• Single particle structure reconstruction
• Semi-quantitative to quantitative analyses

Training in electron microscopy techniques

Equipments

• Scanning Electron Microscope (SEM - Quanta FEG from FEI)
• Transmission Electron Microscope (TEM - Tecnai 10 100kV from FEI)
• Cryogenic Transmission Electron Microscope (cryo-TEM/Talos FEG 200kV from TFS, equipped with Falcon III camera)